Defining Primary Stain And Counter Stain
Order ID 53563633773 Type Essay Writer Level Masters Style APA Sources/References 4 Perfect Number of Pages to Order 5-10 Pages Description/Paper Instructions
Defining Primary Stain And Counter Stain
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Microbiology Lab Packet #2
Think of these lab packets as homework for the lab part of the class. They are designed to help prepare you for the departmental lab exams. To answer these questions you will need to use both the lab workbook as well as your text book. In addition to these questions, you will need to turn in the signed data sheets from your workbook.
- Describe the molecular basis behind Gram staining. What part of the bacteria is stained with the primary stain, what part is stained with the counter stain? Why do we wash alcohol over the slide before the counter stain?
- Describe in great detail the steps in performing a Gram stain. Include incubation times. Define primary stain and counter stain. In the Gram stain, what dye is primary stain, which is the counter stain?
Special Stains (Capsule Ex. 3.9, Endospore Ex. 3-10, Flagella Ex. 3.12)
- What is the stain is shown in the picture below? What is stained green, what is stained red?
- Describe in great detail the steps in performing an endospore stain. Include incubation times.
- What does it mean to be Gram positive, Gram negative?
- Label the different flagellum arrangements seen below.
- Describe the negative staining technique and determine when it is used.
Colony Morphology Ex 2.2
- We can learn some information about bacteria by just looking at its growth on an agar plate. What is a small isolated growth of bacteria on an agar plate called and what can it tell us about the bacteria?
Micro lab packet 2
Kirby-Bauer Method Ex 7.3
- Describe in great detail the technique used to determine bacteria sensitivity to antibiotics?
- What is the type of media used in the Kirby-Bauer test and why is it used?
Describe what is observed for a bacteria that is sensitive to antibiotics in a Kirby-Bauer test, and to a bacteria that is not sensitive to antibiotics.
- How does the antibiotic get from the disk into the agar?
Does the agar have an antibiotic beyond the zone of inhibition?
- Define the MIC and explain how it is used to determine if a bacteria is resistant or sensitive to the antibiotic.
Is the presence of a clear zone around the disc enough evidence to determine antibiotic resistance?
Micro lab packet 2
Disinfectants Ex 2.14
- Compare your results with the class data.
Which germicide was most effective and at what concentration?
Which was the least effective? Defend your choices.
- Which organism seemed to be most resistant to the germicides?
- What purpose did the four controls serve? Be specific.
Include Completed Data sheets for:
- Data Sheet 3-7 (pages 201-202) Sketch & id whether Gram + or Gram – and Questions 1-2
- Data Sheet 3-9 (pages 213-214) Sketch capsuled stained bacteria; Questions 1 & 2
- Data Sheet 3-10 (pages 219-220) Sketch Endospore Stained bacteria; Questions 1-4
- Data Sheet 3-12 (pages 227-228) Sketch Flagella Stained bacteria; Questions 1 & 3
- Data Sheet 2-14 (pages 139-140) Questions 1-3
- Data Sheet 7-3 (pages 535-536) Questions 1-7
Gram Staining 3.7
- What is the difference between disinfectants and antiseptics?
RUBRIC
QUALITY OF RESPONSE NO RESPONSE POOR / UNSATISFACTORY SATISFACTORY GOOD EXCELLENT Content (worth a maximum of 50% of the total points) Zero points: Student failed to submit the final paper. 20 points out of 50: The essay illustrates poor understanding of the relevant material by failing to address or incorrectly addressing the relevant content; failing to identify or inaccurately explaining/defining key concepts/ideas; ignoring or incorrectly explaining key points/claims and the reasoning behind them; and/or incorrectly or inappropriately using terminology; and elements of the response are lacking. 30 points out of 50: The essay illustrates a rudimentary understanding of the relevant material by mentioning but not full explaining the relevant content; identifying some of the key concepts/ideas though failing to fully or accurately explain many of them; using terminology, though sometimes inaccurately or inappropriately; and/or incorporating some key claims/points but failing to explain the reasoning behind them or doing so inaccurately. Elements of the required response may also be lacking. 40 points out of 50: The essay illustrates solid understanding of the relevant material by correctly addressing most of the relevant content; identifying and explaining most of the key concepts/ideas; using correct terminology; explaining the reasoning behind most of the key points/claims; and/or where necessary or useful, substantiating some points with accurate examples. The answer is complete. 50 points: The essay illustrates exemplary understanding of the relevant material by thoroughly and correctly addressing the relevant content; identifying and explaining all of the key concepts/ideas; using correct terminology explaining the reasoning behind key points/claims and substantiating, as necessary/useful, points with several accurate and illuminating examples. No aspects of the required answer are missing. Use of Sources (worth a maximum of 20% of the total points). Zero points: Student failed to include citations and/or references. Or the student failed to submit a final paper. 5 out 20 points: Sources are seldom cited to support statements and/or format of citations are not recognizable as APA 6th Edition format. There are major errors in the formation of the references and citations. And/or there is a major reliance on highly questionable. The Student fails to provide an adequate synthesis of research collected for the paper. 10 out 20 points: References to scholarly sources are occasionally given; many statements seem unsubstantiated. Frequent errors in APA 6th Edition format, leaving the reader confused about the source of the information. There are significant errors of the formation in the references and citations. And/or there is a significant use of highly questionable sources. 15 out 20 points: Credible Scholarly sources are used effectively support claims and are, for the most part, clear and fairly represented. APA 6th Edition is used with only a few minor errors. There are minor errors in reference and/or citations. And/or there is some use of questionable sources. 20 points: Credible scholarly sources are used to give compelling evidence to support claims and are clearly and fairly represented. APA 6th Edition format is used accurately and consistently. The student uses above the maximum required references in the development of the assignment. Grammar (worth maximum of 20% of total points) Zero points: Student failed to submit the final paper. 5 points out of 20: The paper does not communicate ideas/points clearly due to inappropriate use of terminology and vague language; thoughts and sentences are disjointed or incomprehensible; organization lacking; and/or numerous grammatical, spelling/punctuation errors 10 points out 20: The paper is often unclear and difficult to follow due to some inappropriate terminology and/or vague language; ideas may be fragmented, wandering and/or repetitive; poor organization; and/or some grammatical, spelling, punctuation errors 15 points out of 20: The paper is mostly clear as a result of appropriate use of terminology and minimal vagueness; no tangents and no repetition; fairly good organization; almost perfect grammar, spelling, punctuation, and word usage. 20 points: The paper is clear, concise, and a pleasure to read as a result of appropriate and precise use of terminology; total coherence of thoughts and presentation and logical organization; and the essay is error free. Structure of the Paper (worth 10% of total points) Zero points: Student failed to submit the final paper. 3 points out of 10: Student needs to develop better formatting skills. The paper omits significant structural elements required for and APA 6th edition paper. Formatting of the paper has major flaws. The paper does not conform to APA 6th edition requirements whatsoever. 5 points out of 10: Appearance of final paper demonstrates the student’s limited ability to format the paper. There are significant errors in formatting and/or the total omission of major components of an APA 6th edition paper. They can include the omission of the cover page, abstract, and page numbers. Additionally the page has major formatting issues with spacing or paragraph formation. Font size might not conform to size requirements. The student also significantly writes too large or too short of and paper 7 points out of 10: Research paper presents an above-average use of formatting skills. The paper has slight errors within the paper. This can include small errors or omissions with the cover page, abstract, page number, and headers. There could be also slight formatting issues with the document spacing or the font Additionally the paper might slightly exceed or undershoot the specific number of required written pages for the assignment. 10 points: Student provides a high-caliber, formatted paper. This includes an APA 6th edition cover page, abstract, page number, headers and is double spaced in 12’ Times Roman Font. Additionally, the paper conforms to the specific number of required written pages and neither goes over or under the specified length of the paper. GET THIS PROJECT NOW BY CLICKING ON THIS LINK TO PLACE THE ORDER
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